DNA

Part:BBa_K1039026:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


Bxb1 Invertible Medium Strength Promoter Switch Test Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 749
    Illegal NheI site found at 949
    Illegal NheI site found at 972
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 1872
    Illegal AgeI site found at 1984
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74
    Illegal BsaI site found at 781
    Illegal BsaI.rc site found at 1263


Design Notes

Note that there is an NheI site on the prefix side of the switch before the attP site. This was placed there as part of an early design of our switch, but does not contribute to functionality.

Source

The parts were synthesized from BioBasic Inc or from the parts registry

References